Phosphatidate biosynthesis in mitochondrial subfractions of rat liver
نویسندگان
چکیده
منابع مشابه
Phosphatidate biosynthesis in mitochondrial subfractions of rat liver.
1. After conventional fractionation of rat liver homogenates in 0.88m-sucrose the mitochondrial fraction was subjected to short-term water lysis followed by separation of the resulting membrane preparations. 2. Phosphatidate formation was measured in all subcellular fractions and subfractions and was compared with the distribution of succinate dehydrogenase, monoamine oxidase, rotenone-insensit...
متن کاملTranslocation to rat liver mitochondria of phosphatidate phosphohydrolase.
When a particle-free supernatant fraction from rat liver was incubated at 37 degrees C with mitochondria and oleate, some of the enzyme phosphatidate phosphohydrolase (PAP), initially present in the particle-free supernatant, was recovered, after the incubation, bound to mitochondria. This translocation of PAP from cytosol to mitochondria was stimulated by oleate or palmitate in a similar fashi...
متن کاملMitochondrial turnover in animal cells. Half-lives of mitochondria and mitochondrial subfractions of rat liver based on [14C]bicarbonate incorporation.
The half-lives of proteins of mitochondria and a well characterized set of mitochondrial subfractions have been estimated using two methods based on incorporation of a minimally reutilized amino acid precursor, [‘‘Clbicarbonate. The apparent average rate constants of protein degradation were measured on rat liver homogenate, mitochondria, and distinct outer compartment, inner membrane, and matr...
متن کاملThe lipid fluidity of rat liver membrane subfractions.
1. The lipid fluidity of three major rat liver plasma-membrane subfractions, as well as Golgi apparatus and endocytic fractions, was assessed with a fatty acid spin probe by using e.s.r. techniques. 2. The sinusoidal (blood-facing) plasma-membrane subfraction was the most fluid of the three plasma-membrane regions. Fractions originating from the bile-canalicular and contiguous (lateral) regions...
متن کاملPolyribonucleotide synthesis by subfractions of microsomes from rat liver.
1. The microsome fraction of rat liver has been fractionated and the ability of the fractions to incorporate ribonucleotides into polyribonucleotides has been studied. Activity was found in the rough-surfaced vesicle (light) fraction and in the free-ribosome fraction and this latter activity has been examined. 2. The free-ribosome fraction contains ribosome monomers, dimers and trimers together...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1969
ISSN: 0306-3283
DOI: 10.1042/bj1130429